Plasmid_Backbone

Part:BBa_K5300023:Design

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-23)


pBBR1MCS-2, a kind of plasmid vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5174
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2244
    Illegal NotI site found at 5161
    Illegal NotI site found at 5180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5174
    Illegal BglII site found at 1500
    Illegal BamHI site found at 33
    Illegal XhoI site found at 84
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5174
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5174
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5189
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 553
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 4520
    Illegal AgeI site found at 4680
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1924


Design Notes

The compatibility.


Source

artificial synthesized from Kovach et al.1995


References